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KMID : 1234920190190020089
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Journal of Korean Oriental Association for Study of Obesity
2019 Volume.19 No. 2 p.89 ~ p.96
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Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells
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Kim Ji-Hyun
Kim Min-Jeong
Park Hye-Sook
Kim Young-Sil
Cho Eun-Ju
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Abstract
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Objectives: We investigated whether membrane free stem cell extract from adipose tissue (MFSCE) has anti-diabetic effect.
Methods: To determine glucose uptake effect of MFSCE, we carried out glucose uptake assay in 3T3-L1 adipocytes. The regulatory mechanisms of MFSCE on glucose uptake were examined by Western blot analysis.
Results: When MFSCE was treated to adipocytes at the concentration of 0.5, 1, 2.5, and 5 μg/mL, 2-deoxyglucose-6-phosphate uptake was elevated approximately 1.8-fold compared to cells not treated with MFSCE. It indicated that MFSCE enhances glucose uptake in 3T3-L1 adipocytes. In addition, MFSCE reduced phosphorylation of insulin receptor substrate-1 at serine 307 and induced Akt and glucose transporter 4 protein expressions that were related to insulin signaling. Furthermore, MFSCE regulated adenosine monophosphate-activated protein kinase (AMPK) pathway by increases of increase phosphorylation of AMPK and acetyl-CoA carboxylase that were related to AMPK pathway.
Conclusions: These results indicated that MFSCE promotes glucose uptake via modulation of insulin signaling and AMPK pathway. Therefore, MFSCE could be a promising agent for treatment of diabetes mellitus.
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KEYWORD
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AMP-activated protein kinases, Diabetes mellitus, Membrane free stem cell extract, 3T3-L1 cells
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